T-LAB Academy

EDUCATION AND TRAINING
1 Mar 2005–1 Aug 2010 Licenciature in Biological Science, Pontificia Universidad Católica de Chile, Santiago (Chile), EQF level 6
1 Mar 2011–1 Aug 2015 Doctor in Medical Science (MD PhD), Pontificia Universidad Católica de Chile, Santiago (Chile), EQF level 8
1 Oct 2015–31Dec 2016 Post doctoral fellowship in dermatovenerology, Juntendo University, Tokyo (Japan)
25 Aug 2018 / 20 Recognition of medical degree (#CZ03618), Charles University First Faculty of Medicine, Prague (Czech Republic)
1 Mar 2019 Master (Level 7Certificate) in Injectables and Aesthetic Medicine, Acquisition Aesthetics - Barts School of Medicine, London (United Kingdom) - June 2019: post graduate diploma Combined Foundation and Advanced Botox® and Dermal Fillers, EQF level 7
1 May 2019–1Jul 2019 Certificate in advanced skin therapies, Emma Coates Aesthetic Training, Microneedling, Skin Peel, Carboxytherapy and Mesotherapy training, EQF level 4
30 Jul 2019 Minister of Education, Osvědčení o získání profesní kvalifikace 69-035-M / 69-032-M / 69-030-M
Sept 2019 European Accreditation Council for Continuing Medical Education (EACCME®) 21 European Union of Medical Specialists credits
March 2021 Osvědčení – kvalifikace v Ceske republice, NO/UKRUK/750/2020/Pv
July 2021 Profesional Certificate of General Dermatology, Bond University, Australia

WORK EXPERIENCE
1 Mar 2012–1Nov 2012 Staff Associate - Clinician, Columbia University, Internal Medicine Physicians and Surgeons, New York (United States)
1 Aug 2014–Present Medical and Scientific Consultant, Fresenius Kabi, Bad Homburg (Germany)
1 Nov 2017–1Nov 2018 Medical advisor, Estheticon, Prague (Czechia)
1 Jan 2017–Present Senior researcher, Czech Academy of Science, Prague (Czechia)
1 Feb 2019–Present Medical researcher, Zepter- Biotron CZ, Prague (Czechia)
1 Sept 2019–2020 Esthetic medicine doctor, Laserová dermatologická klinika ALTOS, Prague (Czechia)
1 Apr 2020–Sept Esthetic medicine doctor, Swiss Aesthetic, Prague (Czechia)
1 May 2020–Dec 2021 Dermatologist, ABClinic Art & Beauty
April 2021–Dec 2021 Aesthetic medicine doctor, Beauty Shape Clinic
1 Jan 2022–Present Aesthetic medicine doctor, ALR Aesthetics s.r.o, Prague (Czechia)

ADDITIONAL INFORMATION
Industry Certifications

• Merz: Belotero, Bocouture, Ulthera and Radiesse
• IBSA: Profhilo and Aliaxin
• Sinclair: Ellanse and Silhouette threads Allergan: Juvederm and Botox Galderma: Restylane
• NyuPharma: Hyamira
• Hyalual: Xela rederm and Alexa Teoxane: Teosyal
• Deka Laser
• Jesys Laser and RF devices Alma Laser
• Thermage FX and Liposonix Reneve
• Aptos

Career highlights

• Over 100 positive reviews: Estheticon, Whatclinic and google
• Cooperation Bulovka Hospital
• Medical trainer for aesthetic medicine doctors
• Featured in Marie Claire Magazine, Lui, OKO, Teta Magazine, Blesk, Vogue, among others
• High profile patients

AFFILIATIONS

• Czech Academy of Dermatovenerology #551
• Česká lékařská společnost Jana Evangelisty Purkyně, z.s. Registrace dermatologa International Peeling Society
• WOSIAM
• American Academy of Aesthetic Medicine

Conferences

• 1. Leguina A.*, Vio C., Velarde V.. EFFECT OF TGF-Β1 AND ANGIOTENSINE II IN THE EMT AND THE RENAL FIBROSIS PROGRESSION. XXV Annual Reunion Chilean Society of Physiological Science. Santa Cruz, Chile October 23-26 th 2010 (poster)
• Baquedano C., Ramírez A., Curoto C., Santander F., Torres E., Archiles S., Arellano G., Celos O., Vergara F., Romero V., Gallardo., Leguina-Ruzzi A*. ¿ES NECESARIO UN CODIGO DE ETICA DE LOS ESTUDIANTES UNIVERSITARIOS? VI Congreso Internacional de bioética en Iberoamérica, Chile October 15 th 2013 (poster)
• Leguina-Ruzzi A.*, Pereira-Flores K., Decap V., Pereira J., Velarde V., Sáez CG. PALMITIC ACID, IL-6 AND INSULIN COMBINED INDUCE ENDOTHELIAL DAMAGE THAT IS REVERTED BY A RHOA/ROCK INHIBITOR, XXVIII Annual Reunion Chilean Society of Physiological Science. Termas de Catillo, Chile September 24-27th 2013 (poster)
• Leguina-Ruzzi A., Pereira-Flores K., Decap V., Pereira J., Velarde V., Saez CG.. PALMITIC ACID, IL-6 AND INSULIN COMBINED INDUCE ENDOTHELIAL DYSFUNCTION THROUGH RHOA/ROCK ACTIVATION, 18th International Vascular Biology Meeting. Kyoto, Japan April 14-17th 2014 (poster)
• Leguina-Ruzzi A*. NEW PLASMATIC MARKERS OF METABOLIC SYNDROME IN YOUNG ADULTS: A POSSIBLE DIAGNOSIS OF AN EARLY ENDOTHELIAL DYSFUNCTION. Young Investigator's Symposium, XVIII meeting of Clinical Chemistry. Santiago, Chile August 4-6t th 2014 (oral)
• Leguina-Ruzzi A.*, Pereira-Flores K., Valderas JP., Acevedo M., Pereira J., Velarde V., Saez CG. MOLÉCULAS ELEVADAS EN SUJETOS JÓVENES CON SÍNDROME METABÓLICO: IL-6, INSULINA Y ÁCIDO PALMÍTICO, INDUCEN DISFUNCIÓN ENDOTELIAL A TRAVÉS DE LA ACTIVACIÓN DE RHOA/ROCK. LI Chilean Meeting of Cardiology and Cardiovascular Surgery, Viña del Mar, Chile November 29- December 2nd 2014. (oral)
• Leguina-Ruzzi A*. THE MOLECULAR ASPECTS OF PARENTERAL NUTRITION: THERAPEUTIC TARGETS OF GLUTAMINE. Nutrition Course for Clinical Pharmacists, Chilean Society of Intensive Care Medicine, Santiago, Chile June 12nd, 2015. (Oral: plenary lecture sponsored by Fresenius-Kabi)
• Pereira J., Sáez CG., Pereira-Flores K., Leguina-Ruzzi A., Massardo T., Mezzano D. DAÑO ENDOTELIAL INDUCIDO POR COCAINA: BIODISPONIBILIDAD DE OXIDO NITRICO Y EFECTO DE LA ATORVASTATINA. XIX Chilean Meeting of Hematology and Transfusional Medicine. Puerto Varas, Chile October 1-4t th2014
• Pereira-Flores K., Leguina-Ruzzi A., Sáez CG., Mezzano D., Pereira J*. PARTICIPACIÓN DEL RECEPTOR SIGMA-1 EN ACTIVACIÓN DE RHOA/RHO KINASA Y DISFUNCIÓN ENDOTELIAL PROVOCADAPOR COCAÍNA. LI Chilean Meeting of Cardiology and Cardiovascular Surgery. Viña del Mar, Chile November 29- 2nd December 2014
• Leguina-Ruzzi A.*, Pereira J., Pereira-Flores K., Valderas JP., Acevedo M., Mezzano D., Velarde V., Sáez C. SUJETOS CON SÍNDROME METABÓLICO, PRE HIPERTENSOS Y SIN DIABETES TIPO 2 PRESENTAN SIGNOS DE DISFUNCIÓN ENDOTELIAL ASOCIADO A ACTIVACIÓN DE RHOA/ROCK. I Meeting of the Chilean Society of Hypertension. Santiago, Chile November 5-9th September 2015 (poster)
• Leguina-Ruzzi A.*, Peñaloza E., Pereira J., Romero D., Roa JC., Mezzano D., Sáez C., Velarde V. STUDIES ON THE ROLE OF RHOA-RHO KINASE ACTIVATION IN AN ANIMAL MODEL OF METABOLIC SYNDROME. XXV Annual Reunion Chilean Society of Physiological Science. Copiapo, Chile September 22-25 th 2015 (oral)
• Leguina-Ruzzi A.*, Koga T., Okuno T., Saeki K., Ohba M., Hara T., Yokomizo T. BLT2 PROTECTS SKIN FROM THE DAMAGE CAUSED BY TYPE 2 DIABETES HYPERGLYCEMIA BY INCREASING NO PRODUCTION THROUGH THE NNOS EXPRESSION. Keystone Symposium G Protein- Coupled Receptors: Structure, Signaling and Drug Discovery (B3). Keystone, USA February 21-25 th 2016 (poster).
• Leguina-Ruzzi A*, Pruchova P, Holendova B, Jezek P, Jaburek M. PLA2γ ABLATION ALTERS GLUCOSE HOMEOSTASIS AND INSULIN SECRETATION IN RESPONSE TO FATTY ACIDS. SfRBM's 24th Annual Meeting Program and Abstracts November 29 - December 2, 2017 Hilton Baltimore Baltimore, MD USA.
• Jaburek M, Pruchova P, Chronowska E, Leguina-Ruzzi A, Jezek P. PHOSPHOLIPASE IPLA2γ PARTICIPATES IN CELLULAR ANTIOXIDANT AND ANTI-INFLAMMATORY PROTECTION IN VIVO. SfRBM's 24th Annual Meeting Program and Abstracts November 29 - December 2, 2017 Hilton Baltimore Baltimore, MDUSA.
• Pruchova P, Leguina-Ruzzi A, Galkin A, Jezek P, Jaburek M. ANTIOXIDANT ACTIVITY OF CALCIUM-INDEPENDENT PHOSPHOLIPASE A2Γ IN BRAIN MITOCHONDRIA. November 2018. Free Radical Biology and Medicine 128:S86. EBEC conference, Budapest, Hungary.
• Jezek P, Holendova B, Leguina-Ruzzi A, Pruchova P, Jaburek M. REDOX-ACTIVATED MITOCHONDRIAL IPLA2Γ PARTICIPATES IN FATTY ACID-STIMULATED INSULIN SECRETION. November 2018. Free Radical Biology and Medicine 128:S51. EBEC conference, Budapest, Hungary.

Publications

• 1. Leguina-Ruzzi A, Ortiz R. Current Evidence for the Use of Smoflipid® Emulsion in Critical Care Patients for Parenteral Nutrition. Critical Care Research and Practice. 2018.
• Leguina-Ruzzi A, Ortiz R, Velarde V. The Streptozotocin-high fat diet induced diabetic mouse model exhibits severe skin damage and alterations in local lipid mediators. Biomedical Journal. 2018.
• Leguina-Ruzzi A, Cariqueo M, Galvez R. Three years after REDOX study: what we have learned about the use of Glutamine in ICU patients?. TAAC. 2016.
• Leguina-Ruzzi A. Cardioprotective and anti-inflammatory effects of glutamine in parental nutrition. NJCC. 2017 Jan; 25(1):6-8.
• Leguina-Ruzzi A, Cariqueo M. (2016). Glutamine: a conditionally essential amino acid with multiple biological functions. Superfood and Functional Food - Development of Superfood and its Role in Medicine. Shiomi N, Waisundara VY (Eds.) Rijeka, Croatia: InTech.
• Leguina-Ruzzi A, Valderas JP. BLT2 expression improves skin integrity and protects from alterations caused by hyperglycemia in type 2 diabetes. Dermatoendocrinol. 2016 Dec; 9(1): 1-12.
• Cardenas H, Leguina-Ruzzi A, Baquedano C, Ramirez A, Santander F, Torres E, Archiles S, Arellano G, Vergara F, Romero V, Gallardo P, Arbiter R, Orihuela P. Student View on Ethical Aspects of Undergraduate Research at a Chilean Public University. Contribuciones Científicas y Tecnológicas. 2016 Nov; 141(41): 77-82.
• Leguina-Ruzzi A. Bases Moleculares de la Disfunción Endotelial en Síndrome Metabólico: Estudio de la vía Rho/ROCK como posible eje de señalización. ISBN: 978-3-639-78631. Spain: Editorial Académica Española; (2016).Languages
• Leguina-Ruzzi A. A commentary on the 2015 Canadian Clinical Practice Guidelines in glutamine supplementation to parenteral nutrition. Critical Care. 2016 Jan 8;20:7. doi: 10.1186/s13054-015-1175- 3.
• Leguina-Ruzzi A, Pereira J, Pereira-Flores K, Valderas JP, Mezzano D, Velarde V, Sáez CG. Increased RhoA/Rho-Kinase Activity and Markers of Endothelial Dysfunction in Young Adults Subjects with Metabolic Syndrome. Metab Syndr Relat Disord. 2015 Nov;13(9): 373-380. 10- 1089/met.2015.0061
• Leguina-Ruzzi A. Therapeutic targets of glutamine in parental nutrition: a medical science review. Inter J PrevTreat. 2015;4:34-9.
• Hernández-Salinas R, Decap V, Leguina-Ruzzi A, Cáceres P, Perez D, Urquiaga I, Iturriaga R, Velarde V. Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet. Biol Res. 2015 Sept 30;48:53. doi: 101186/s40659-015-0045-4.
• Leguina-Ruzzi A. An Induced Disequilibrium Between the AT1 and B2R Impairs the Trophoblastic Motile Capacity: a Cellular Insight of Preeclampsia. Basic Sciences Of Medicine. 2015; 4 (1): 5-11.

Under review

• Leguina-Ruzzi A, Ortiz R, Velarde V. The 12-HHT/BLT2/NO axis is associated to the wound healing and skin condition in different glycemic states. Biomedical Journal (BMC)
• Leguina-Ruzzi A, Tonks SK, Raichamura K, Kwabi S, Leitner C. reatment of Non-Atopic Dermatitis with Polarized UV Free Polychromatic Light: A Case Report. Dermatitis
• Leguina-Ruzzi A, Peñaloza E, Hernandez MP, Velarde V, Pereira J, Mezzano D, Sáez C. Metabolic syndrome induces endothelial dysfunction through the RhoA/ROCK activation. Circulation

Seminars

• July 29, 2016: 2do Curso Farmacoterapia en Medicina Intensiva: Glutamine in ICU Patient
• Feb 16, 2015: Pusan National University School of Medicine: Signs of Endothelial Dysfunction in Young Asymptomatic Patients with Metabolic Syndrome: Association with RhoA/ROCK.
• April 14, 2013: Tokio Medical and Dental University: In vitro study of Endothelial Dysfunction in Metabolic Syndrome: RhoA/ROCK as cell signaling hub.
• Trainings for Czech doctors in Amaryllis Clinic, BeautyShape, Abclinic among others. Dermal fillers, botulinum toxin, mesotherapy, chemical peelings and redermalization techniques.

Academic and teaching experience

• Assistant Lecturer: course Molecular Biology Of The Cell (BIO141C) 2nd Semester 2007 Professor in charge: Francisca Bronfman PhD
• Assistant Lecturer: course Biochemistry and Cell Biology Lab (BIO297C) 1st Semester 2008 Professor in charge: Juan Larraín PhD
• 3. Assistant Lecturer: course Biochemistry and Cell Biology Lab (BIO297C) 1st Semester 2009 Professor incharge: Alejandra Alvarez PhD
• Assistant Lecturer: course Molecular Biology Of The Cell (BIO141C) 1st Semester 2010 Professor incharge: Alejandro Erices PhD
• Assistant Lecturer: course Microbiology and Immunology (BIO145G) 1st Semester 2010 Professor incharge: Carolina Serrano PhD
• Assistant Lecturer: course Microbiology and Immunology (BIO145G) 2nd Semestre 2010 Professor incharge: Carolina Serrano PhD
• Assistant Lecturer: course Microbiology and Immunology (BIO145C) 2nd Semestre 2010 Professor incharge: Monica Vasquez PhD
• Assistant Lecturer: course Topics in Biology (BIO100C) 1st Semestre 2015 Professor incharge: Carolina Serrano PhD
• Assistant Lecturer: course Clinical Micrbiology (BIO145G) 1st Semestre 2015 Professor incharge: Bruno Tesser PhD
• Research unit and biochemical professional practice supervisor 2013-2014 Undergraduate students Maria Paz Hermandez and Estefania Peñaloza
• Faculty of medicine scientific program supervisor 2015 Medical student Cecilia Alarcon

Honours and awards

• SAIAVisiting University Teachers and Researchers, National Scholarship Programme of the Slovak Republic - SAIA, no, 2017
• Post doctoral fellow. Research Institute for Disease of Old Age, Japan, 2015
• Doctoral Dissertation Fellowship. CONICYT,2014
• Grant "Encuentros Chile Global 2014" Young Leaders. VRI, 2014
• Support for the Organization of Seminars by Doctorates. VRI, 2014
• Best Research Work Prevention of the Cardiovascular Pathologies. Chilean Cardiology Foundation, 2014
• Support Grant for PhD Thesis. DRI Faculty of Medicine, Universidad Católica de Chile , 2014
• Support Grant for PhD Thesis. CONICYT, 2014
• Grant for IVBM Meeting Attendance. VRI Pontificia Universidad Católica de Chile, 2014
• Grant PhD studies for tuition fees and maintenances. CONICYT, 2014
• Best Panel Presentation, Young Researcher Award. Chilean Society of Physiological Science, 2013
• Best Undergraduate Thesis. Faculty of Biological Science, Pontificia Universidad Católica de Chile, 2010
• WhatClinic Patient Service Awards 2019 and 2020